Analytical Ultracentrifugation of Polymers and Nanoparticles by Walter Maechtle

By Walter Maechtle

This e-book is split into chapters masking instrumentation, sedimentation pace runs, density gradient runs, software examples and destiny advancements. particularly, the distinct program bankruptcy demonstrates the flexibility and tool of AUC by way of many fascinating and significant business examples. hence the booklet concentrates on useful elements instead of info of centrifugation conception.

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2mm wide slit arranged perpendicularly to the radius of the rotor. The slit picks out a beam from the entering parallel monochromatic light. This is simply created by using a stabilized incandescent lamp as a light source, and a monochromatic light filter (λ = 546nm) after the condensing lens. The intensity I of the beam, which is reduced by light scattering of the latex particles inside the measurement cell, according to Mie’s light scattering theory, is registered by the photomultiplier and recorded as a function of the running time t.

4 Detectors 37 sometimes one or more of the scans are shifted vertically by one or two integer numbers, for example, ΔJ = ±1, ±2, etc. However, this shifting can be corrected easily. Furthermore, if a sample shows too much turbidity, the fringes on the CCD camera monitor appear faintly, and it could be possible that the Fourier transform analysis fails. 3 Schlieren Optics The Schlieren optics setup (as described above in Sect. 2 and Fig. 3) is similar to the setup of the Rayleigh interferometer but has a phase plate (or knife-edge) in the focus of the condenser lens as an additional element.

Whenever the magnet passes the sensing device placed at the bottom of the vacuum chamber, the magnet induces an electrical signal. The position of the cell, and the sector of the cell of interest with respect to the position of the magnet on the rotor is known, and therefore the time delay from the magnet passing the sensing device until the lamp has to flash can be calculated within the XL-A/I computer, using the known rotor speed. The duration of a single flash is about 2–3 μs, which is sufficient to create data from only one sector of the double-sector cell at once.

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